Best Practices for Peptide Storage and Handling

Proper storage and handling of research peptides is crucial for maintaining their integrity and ensuring reproducible experimental results. This guide covers essential practices every researcher should follow.

Storage Guidelines

Lyophilized Peptides - Store at -20°C or below - Protect from light and moisture - Keep in original sealed vials until use - Typical stability: 24-36 months when stored properly

Reconstituted Peptides - Store at 2-8°C for short-term use (up to 2-4 weeks) - For longer storage, aliquot and freeze at -20°C - Avoid repeated freeze-thaw cycles - Use within recommended timeframe

Reconstitution Protocol

1. **Allow to warm**: Let the vial reach room temperature before opening
2. **Choose solvent**: Use sterile water, bacteriostatic water, or appropriate buffer
3. **Add solvent slowly**: Direct liquid down the side of the vial
4. **Mix gently**: Swirl or rotate - never vortex vigorously
5. **Allow to dissolve**: Wait for complete dissolution before use

Common Mistakes to Avoid

• Opening cold vials (causes condensation)
• Using incorrect solvents
• Storing reconstituted peptides at room temperature
• Contaminating stock solutions

Quality Indicators

Signs of peptide degradation: - Cloudiness or precipitation - Color changes - Reduced biological activity - Unusual odor

Following these guidelines will help ensure your peptides maintain their quality throughout your research program.