Understanding Peptide Purity: A Guide to HPLC Analysis
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Understanding Peptide Purity: A Guide to HPLC Analysis

15 December 2025

High-Performance Liquid Chromatography (HPLC) is the gold standard for peptide purity analysis in research laboratories worldwide. This analytical technique separates, identifies, and quantifies components in a mixture, making it invaluable for quality control.

How HPLC Works

HPLC operates by pumping a sample mixture through a column packed with a solid adsorbent material. Different components in the sample interact differently with the adsorbent, causing them to elute at different times. By measuring the retention time and peak area, we can determine both the identity and quantity of peptides present.

Why Purity Matters

For research applications, peptide purity directly impacts experimental reproducibility. Impurities can: - Interfere with binding assays - Cause unexpected biological responses - Lead to inconsistent results between experiments

Our Standards

At Peptidyne, we maintain strict purity standards: - ≥98% purity for standard research grade - ≥99% purity for premium research applications - Full Certificate of Analysis with every order

Every batch undergoes rigorous HPLC testing before release, ensuring you receive only the highest quality peptides for your research.

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